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"Biglar (, also Romanized as Bīglar; also known as Beglar) is a village in Quchan Atiq Rural District, in the Central District of Quchan County, Razavi Khorasan Province, Iran. At the 2006 census, its population was 525, in 129 families. References Populated places in Quchan County "
"The schematic of a multifocal plane microscope. Multifocal plane microscopy (MUM) or Multiplane microscopy or Biplane microscopy is a form of light microscopy that allows the tracking of the 3D dynamics in live cells at high temporal and spatial resolution by simultaneously imaging different focal planes within the specimen. In this methodology, the light collected from the sample by an infinity-corrected objective lens is split into two paths. In each path the split light is focused onto a detector which is placed at a specific calibrated distance from the tube lens. In this way, each detector images a distinct plane within the sample. The first developed MUM setup was capable of imaging two distinct planes within the sample. However, the setup can be modified to image more than two planes by further splitting the light in each light path and focusing it onto detectors placed at specific calibrated distances. Another technique called multifocus microscopy (MFM) uses diffractive Fourier optics to image up to 25 focal planes. Presently, MUM setups are implemented that can image up to four distinct planes. Introduction Fluorescence microscopy of live cells represents a major tool in the study of trafficking events. The conventional microscope design is well adapted to image fast cellular dynamics in two dimensions, i.e., in the plane of focus. However, cells are three-dimensional objects and intracellular trafficking pathways are typically not constrained to one focal plane. If the dynamics are not constrained to one focal plane, the conventional single plane microscopy technology is inadequate for detailed studies of fast intracellular dynamics in three dimensions. Classical approaches based on changing the focal plane are often not effective in such situations since the focusing devices are relatively slow in comparison to many of the intracellular dynamics. In addition, the focal plane may frequently be at the wrong place at the wrong time, thereby missing important aspects of the dynamic events. =Implementation= MUM can be implemented in any standard light microscope. An example implementation in a Zeiss microscope is as follows. A Zeiss dual- video adaptor is first attached to the side port of a Zeiss Axiovert 200 microscope. Two Zeiss dual-video adaptors are then concatenated by attaching each of them to the output ports of the first Zeiss video adaptor. To each of the concatenated video adaptor, a high resolution CCD camera is attached by using C-mount/spacer rings and a custom-machined camera coupling adaptor. The spacing between the output port of the video adaptor and the camera is different for each camera, which results in the cameras imaging distinct focal planes. It is worth mentioning that there are many ways to implement MUM. The mentioned implementation offers several advantages such as flexibility, ease of installation and maintenance, and adjustability for different configurations. Additionally, for a number of applications it is important to be able to acquire images in different colors at different exposure times. For example, to visualize exocytosis in TIRFM, very fast acquisition is necessary. However, to image a fluorescently labeled stationary organelle in the cell, low excitation is necessary to avoid photobleaching and as a result the acquisition has to be relatively slow. In this regard, the above implementation offers great flexibility, since different cameras can be used to acquire images in different channels. 3D super-resolution imaging and single molecule tracking using MUM A comparison of the depth discrimination of MUM with conventional single plane microscopy. Modern microscopy techniques have generated significant interest in studying cellular processes at the single molecule level. Single molecule experiments overcome averaging effects and therefore provide information that is not accessible using conventional bulk studies. However, the 3D localization and tracking of single molecules poses several challenges. In addition to whether or not images of the single molecule can be captured while it undergoes potentially highly complex 3D dynamics, the question arises whether or not the 3D location of the single molecule can be determined and how accurately this can be done. A major obstacle to high accuracy 3D location estimation is the poor depth discrimination of a standard microscope. Even with a high numerical aperture objective, the image of a point source in a conventional microscope does not change appreciably if the point source is moved several hundred nanometers from its focus position. This makes it extraordinarily difficult to determine the axial, i.e., z position, of the point source with a conventional microscope. More generally, quantitative single molecule microscopy for 3D samples poses the identical problem whether the application is localization/tracking or super-resolution microscopy such as PALM, STORM, FPALM, dSTORM for 3D applications, i.e. the determination of the location of a single molecule in three dimensions. MUM offers several advantages. In MUM, images of the point source are simultaneously acquired at different focus levels. These images give additional information that can be used to constrain the z position of the point source. This constraining information largely overcomes the depth discrimination problem near the focus. The 3D localization measure provides a quantitative measure of how accurately the location of the point source can be determined. A small numerical value of the 3D localization measure implies very high accuracy in determining the location, while a large numerical value of the 3D localization measure implies very poor accuracy in determining the location. For a conventional microscope when the point source is close to the plane of focus, e.g., z0 <= 250 nm, the 3D localization measure predicts very poor accuracy in estimating the z position. Thus, in a conventional microscope, it is problematic to carry out 3D tracking when the point source is close to the plane of focus. On the other hand, for a two plane MUM setup the 3D localization measure predicts consistently better accuracy than a conventional microscope for a range of z-values, especially when the point source is close to the plane of focus. An immediate implication of this result is that the z-location of the point source can be determined with relatively the same level of accuracy for a range of z-values, which is favorable for 3D single particle tracking. Dual objective multifocal plane microscopy (dMUM) Dual objective multifocal plane microscope (dMUM). In single particle imaging applications, the number of photons detected from the fluorescent label plays a crucial role in the quantitative analysis of the acquired data. Currently, particle tracking experiments are typically carried out on either an inverted or an upright microscope, in which a single objective lens illuminates the sample and also collects the fluorescence signal from it. Note that although fluorescence emission from the sample occurs in all directions (i.e., above and below the sample), the use of a single objective lens in these microscope configurations results in collecting light from only one side of the sample. Even if a high numerical aperture objective lens is used, not all photons emitted at one side of the sample can be collected due to the finite collection angle of the objective lens. Thus even under the best imaging conditions conventional microscopes collect only a fraction of the photons emitted from the sample. To address this problem, a microscope configuration can be used that uses two opposing objective lenses, where one of the objectives is in an inverted position and the other objective is in an upright position. This configuration is called dual objective multifocal plane microscopy (dMUM). References External links *Ward Ober Lab web page at UT Southwestern Medical Center. *FandPLimitTool Home page *MUMDesignTool Home page Microscopy Fluorescence techniques Cell imaging Laboratory equipment "
"William Mellors Henry (1890 – 1970) was an American writer and reporter who lived and worked primarily in Los Angeles, California. He was primarily known for his daily Los Angeles Times column, "By the Way", which appeared from 1939 to 1971. Henry also served as technical director for the 1932 Los Angeles Olympic Games, and authored An Approved History of the Olympic Games (G.P. Putnam's Sons, 1948). Henry's parents, Margaret Wendell Henry and Dr. John Quincy Adams ("J.Q.A.") Henry, moved their family to Los Angeles in 1907, where Dr. Henry worked as a temperance advocate and pastor of the First Baptist Church of Los Angeles. Henry graduated from Los Angeles High School in 1909, and accompanied his father on a missionary trip to Australia and New Zealand the following year. He attended the University of Sydney, and eventually enrolled at Occidental College in 1912. He began reporting on sports for the Los Angeles Times the same year, while playing football and track, and performing in the glee club at Occidental until his graduation in 1914. Henry married Corinne Stanton in 1914, with whom he fathered three daughters. In 1919, he briefly left his position at the Times and moved his family to Cleveland, where he worked at an aircraft plant with Glenn L. Martin. He was an aviation enthusiast, and assisted Donald Douglas in establishing the Douglas Aircraft Company in Los Angeles in 1920. From 1920–1926, Henry served as editor of Touring Topics, a membership magazine produced by the Automobile Club of Southern California. Henry worked as a war correspondent for the Times from 1939–1942, and reported on sports, political conventions, and presidential travel as a news broadcaster for Mutual Broadcasting System (MBS), CBS, and NBC throughout his career. He was also the president of the Radio and Television Correspondents' Association (RTCA) from 1947–1948, and chairman of the Political Conventions Committee of the RTCA from 1952–1968. Henry continued to write for the Times until his death in 1970 at age 80. Shortly before his death, Henry was announced as a recipient of the Presidential Medal of Freedom, the highest civilian award in the United States. He is a member of the Occidental College Track and Field Hall of Fame.http://www.oxyathletics.com/halloffame/trackandfield/HOF See also * List of Presidential Medal of Freedom recipients References External links * 1890 births 1970 deaths Journalists from California Occidental College alumni American television sports anchors Los Angeles Times people American television sports announcers Presidential Medal of Freedom recipients American war correspondents of World War II American war correspondents Date of birth missing Place of birth missing Date of death missing Place of death missing "